| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NDE1 (1-110) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NDE1 |
| Gene ID: |
54820 |
| Uniprot ID: |
NDE1_HUMAN |
| Immunogen Region: |
1-110 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NDE1 (NP_060138.1). |
| Immunogen Sequence: |
MEDSGKTFSSEEEEANYWKD LAMTYKQRAENTQEELREFQ EGSREYEAELETQLQQIETR NRDLLSENNRLRMELETIKE KFEVQHSEGYRQISALEDDL AQTKAIKDQL |
| Tissue Specificity | Expressed in the neuroepithelium throughout the developing brain, including the cerebral cortex and cerebellum. |
| Post Translational Modifications | Phosphorylated in mitosis. Phosphorylated in vitro by CDC2. Phosphorylation at Thr-246 is essential for the G2/M transition. |
| Function | Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex. |
| Protein Name | Nuclear Distribution Protein Nude Homolog 1Nude |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-8854518Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeChromosomeCentromereKinetochoreSpindleCleavage FurrowLocalizes To The Interphase And S Phase CentrosomeDuring MitosisPartially Associated With The Mitotic SpindleConcentrates At The Plus Ends Of Microtubules Coincident With Kinetochores In Metaphase And Anaphase In A Cenpf-Dependent MannerAlso Localizes To The Cleavage Furrow During CytokinesisManner |
| Alternative Antibody Names | Anti-Nuclear Distribution Protein Nude Homolog 1 antibodyAnti-Nude antibodyAnti-NDE1 antibodyAnti-NUDE antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
