• Western blot analysis of extracts of various cell lines, using TXNDC9 antibody (STJ116367) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of TXNDC9 in paraffin-embedded Human colon carcinoma using TXNDC9 Rabbit polyclonal antibody (STJ116367) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Human colon carcinoma using TXNDC9 rabbit polyclonal antibody (STJ116367) at dilution of 1:100 (40x lens).
  • Western blot analysis of various lysates using TXNDC9 Rabbit polyclonal antibody (STJ116367) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TXNDC9 antibody (1-110) (STJ116367)

SKU:
STJ116367

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TXNDC9 (1-110) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TXNDC9
Gene ID: 10190
Uniprot ID: TXND9_HUMAN
Immunogen Region: 1-110
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human TXNDC9 (NP_005774.2).
Immunogen Sequence: MEADASVDMFSKVLEHQLLQ TTKLVEEHLDSEIQKLDQMD EDELERLKEKRLQALRKAQQ QKQEWLSKGHGEYREIPSER DFFQEVKESENVVCHFYRDS TFRCKILDRH
Function Significantly diminishes the chaperonin TCP1 complex ATPase activity, thus negatively impacts protein folding, including that of actin or tubulin.
Protein Name Thioredoxin Domain-Containing Protein 9
Atp-Binding Protein Associated With Cell Differentiation
Protein 1-4
Cellular Localisation Cytoplasm
Nucleus
Cytoskeleton
Microtubule Organizing Center
Centrosome
Midbody
Co-Localizes With Beta-Tubulin In The Centrosome
Alternative Antibody Names Anti-Thioredoxin Domain-Containing Protein 9 antibody
Anti-Atp-Binding Protein Associated With Cell Differentiation antibody
Anti-Protein 1-4 antibody
Anti-TXNDC9 antibody
Anti-APACD antibody

Information sourced from Uniprot.org

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