| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NCOA4 |
| Gene ID: |
8031 |
| Uniprot ID: |
NCOA4_HUMAN |
| Immunogen Region: |
1-614 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NCOA4 (NP_005428.1). |
| Immunogen Sequence: |
MNTFQDQSGSSSNREPLLRC SDARRDLELAIGGVLRAEQQ IKDNLREVKAQIHSCISRHL ECLRSREVWLYEQVDLIYQL KEETLQQQAQQLYSLLGQFN CLTHQLECTQNKDLANQVSV CLERLGSLTLKPEDSTVLLF EADTITLRQTITTFGSLKTI QIPEHLMAHASSANIGPFLE KRGCISMPEQKSASGIVAVP FSEWLLGSKPASGYQAPYIP STDPQDWLTQKQTLENSQT |
| Tissue Specificity | Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis. |
| Post Translational Modifications | NCOA4 levels may be regulated via HERC2-mediated ubiquitination leading to proteosomal degradation.HERC2 association is dependent on NCOA4 iron occupancy. |
| Function | Cargo receptor for the autophagic turnover of the iron-binding ferritin complex, playing a central role in iron homeostasis. Acts as an adapter for delivery of ferritin to lysosomes and autophagic degradation of ferritin, a process named ferritinophagy. Targets the iron-binding ferritin complex to autolysosomes following starvation or iron depletion. Ensures efficient erythropoiesis, possibly by regulating hemin-induced erythroid differentiation. In some studies, has been shown to enhance the androgen receptor AR transcriptional activity as well as acting as ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma. Another study shows only weak behavior as a coactivator for the androgen receptor and no alteration of the ligand responsiveness of the AR. Binds to DNA replication origins, binding is not restricted to sites of active transcription and may likely be independent from the nuclear receptor transcriptional coactivator function. May inhibit activation of DNA replication origins, possibly by obstructing DNA unwinding via interaction with the MCM2-7 complex. |
| Protein Name | Nuclear Receptor Coactivator 4Ncoa-4Androgen Receptor Coactivator 70 Kda Protein70 Kda Ar-Activator70 Kda Androgen Receptor CoactivatorAndrogen Receptor-Associated Protein Of 70 KdaFerritin Cargo Receptor Ncoa4Ret-Activating Protein Ele1 |
| Cellular Localisation | Cytoplasmic VesicleAutophagosomeAutolysosomeNucleusChromosome |
| Alternative Antibody Names | Anti-Nuclear Receptor Coactivator 4 antibodyAnti-Ncoa-4 antibodyAnti-Androgen Receptor Coactivator 70 Kda Protein antibodyAnti-70 Kda Ar-Activator antibodyAnti-70 Kda Androgen Receptor Coactivator antibodyAnti-Androgen Receptor-Associated Protein Of 70 Kda antibodyAnti-Ferritin Cargo Receptor Ncoa4 antibodyAnti-Ret-Activating Protein Ele1 antibodyAnti-NCOA4 antibodyAnti-ARA70 antibodyAnti-ELE1 antibodyAnti-RFG antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
