• Immunofluorescence analysis of 293F cells using NCOA4 Rabbit polyclonal antibody (STJ117818) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of K-562 cells using NCOA4 Rabbit polyclonal antibody (STJ117818) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-3 cells using NCOA4 Rabbit polyclonal antibody (STJ117818) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of NCOA4 in paraffin-embedded mouse lung tissue using NCOA4 Rabbit polyclonal antibody (STJ117818) at a dilution of 1:50 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from Rat testis using NCOA4 Rabbit polyclonal antibody (STJ117818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-NCOA4 antibody (1-614) (STJ117818)

SKU:
STJ117818

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCOA4 (1-614) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCOA4
Gene ID: 8031
Uniprot ID: NCOA4_HUMAN
Immunogen Region: 1-614
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-614 of human NCOA4 (NP_005428.1).
Immunogen Sequence: MNTFQDQSGSSSNREPLLRC SDARRDLELAIGGVLRAEQQ IKDNLREVKAQIHSCISRHL ECLRSREVWLYEQVDLIYQL KEETLQQQAQQLYSLLGQFN CLTHQLECTQNKDLANQVSV CLERLGSLTLKPEDSTVLLF EADTITLRQTITTFGSLKTI QIPEHLMAHASSANIGPFLE KRGCISMPEQKSASGIVAVP FSEWLLGSKPASGYQAPYIP STDPQDWLTQKQTLENSQT
Tissue Specificity Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis.
Function Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.
Protein Name Nuclear Receptor Coactivator 4
Ncoa-4
Androgen Receptor Coactivator 70 Kda Protein
70 Kda Ar-Activator
70 Kda Androgen Receptor Coactivator
Androgen Receptor-Associated Protein Of 70 Kda
Ret-Activating Protein Ele1
Alternative Antibody Names Anti-Nuclear Receptor Coactivator 4 antibody
Anti-Ncoa-4 antibody
Anti-Androgen Receptor Coactivator 70 Kda Protein antibody
Anti-70 Kda Ar-Activator antibody
Anti-70 Kda Androgen Receptor Coactivator antibody
Anti-Androgen Receptor-Associated Protein Of 70 Kda antibody
Anti-Ret-Activating Protein Ele1 antibody
Anti-NCOA4 antibody
Anti-ARA70 antibody
Anti-ELE1 antibody
Anti-RFG antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance