| Host: | Goat |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal anti-Nucleolin (500aa C-Term) for use in WB in Human, Mouse, Rat, Bovine, Canine, Chicken, Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep and Simian samples. Datasheet included with dilution recommenda |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-1:5000 |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: | NCL |
| Gene ID: | 4691 |
| Uniprot ID: | NUCL_HUMAN |
| Immunogen Region: | 500aa C-Term |
| Accession Number: | ENSG00000115053 |
| Specificity: | Detects endogenous levels of NCL by Western blot. |
| Immunogen: | Purified recombinant peptide derived from within residues 500 aa to the C-terminus of human NCL produced in E. coli. |
| Immunogen Sequence: | TLQEVFEKATFIKVPQNQNG LQEVFEKATFIKVPQNQNGK QEVFEKATFIKVPQNQNGKS EVFEKATFIKVPQNQNGKSK VFEKATFIKVPQNQNGKSKG FEKATFIKVPQNQNGKSKGY EKATFIKVPQNQNGKSKGYA KATFIKVPQNQNGKSKGYAF ATFIKVPQNQNGKSKGYAFI TFIKVPQNQNGKSKGYAFIE FIKVPQNQNGKSKGYAFIEF |
| Post Translational Modifications | Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. Symmetrically methylated by PRMT5. |
| Function | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. |
| Protein Name | NucleolinProtein C23 |
| Database Links | Reactome: R-HSA-6791226Reactome: R-HSA-9820960 |
| Cellular Localisation | NucleusNucleolusCytoplasmLocalized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas |
| Alternative Antibody Names | Anti-Nucleolin antibodyAnti-Protein C23 antibodyAnti-NCL antibody |
Information sourced from Uniprot.org
