| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NCF4 |
| Gene ID: | 4689 |
| Uniprot ID: | NCF4_HUMAN |
| Immunogen Region: | 1-190 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-190 of human NCF4/p40-phox (NP_038202.2). |
| Immunogen Sequence: | MAVAQQLRAESDFEQLPDDV AISANIADIEEKRGFTSHFV FVIEVKTKGGSKYLIYRRYR QFHALQSKLEERFGPDSKSS ALACTLPTLPAKVYVGVKQE IAEMRIPALNAYMKSLLSLP VWVLMDEDVRIFFYQSPYDS EQVPQALRRLRPRTRKVKSV SPQGNSVDRMAAPRAEALFD FTGNSKLELN |
| Tissue Specificity | Expression is restricted to hematopoietic cells. |
| Function | Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (Probable). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. |
| Protein Name | Neutrophil Cytosol Factor 4Ncf-4Neutrophil Nadph Oxidase Factor 4Sh3 And Px Domain-Containing Protein 4P40-PhoxP40phox |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1236973Reactome: R-HSA-3299685Reactome: R-HSA-4420097Reactome: R-HSA-5668599Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423 |
| Cellular Localisation | CytoplasmCytosolEndosome MembranePeripheral Membrane ProteinCytoplasmic SideMembraneTranslocates To The Membrane Upon Activation By Phorbol Myristate Acetate (Pma) |
| Alternative Antibody Names | Anti-Neutrophil Cytosol Factor 4 antibodyAnti-Ncf-4 antibodyAnti-Neutrophil Nadph Oxidase Factor 4 antibodyAnti-Sh3 And Px Domain-Containing Protein 4 antibodyAnti-P40-Phox antibodyAnti-P40phox antibodyAnti-NCF4 antibodyAnti-SH3PXD4 antibody |
Information sourced from Uniprot.org
