Anti-NCF4 antibody (1-190) (STJ24700)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NCF4 (1-190) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NCF4 |
| Gene ID | 4689 |
| Uniprot ID | NCF4_HUMAN |
| Immunogen Region | 1-190 |
| Immunogen Sequence | MAVAQQLRAESDFEQLPDDV AISANIADIEEKRGFTSHFV FVIEVKTKGGSKYLIYRRYR QFHALQSKLEERFGPDSKSS ALACTLPTLPAKVYVGVKQE IAEMRIPALNAYMKSLLSLP VWVLMDEDVRIFFYQSPYDS EQVPQALRRLRPRTRKVKSV SPQGNSVDRMAAPRAEALFD FTGNSKLELN |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-190 of human NCF4/p40-phox (NP_038202.2). |
Additional Info
| Tissue Specificity | Expression is restricted to hematopoietic cells. |
| Function | Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (Probable). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. |
| Protein Name | Neutrophil Cytosol Factor 4Ncf-4Neutrophil Nadph Oxidase Factor 4Sh3 And Px Domain-Containing Protein 4P40-PhoxP40phox |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1236973Reactome: R-HSA-3299685Reactome: R-HSA-4420097Reactome: R-HSA-5668599Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423 |
| Cellular Localisation | CytoplasmCytosolEndosome MembranePeripheral Membrane ProteinCytoplasmic SideMembraneTranslocates To The Membrane Upon Activation By Phorbol Myristate Acetate (Pma) |
| Alternative Antibody Names | Anti-Neutrophil Cytosol Factor 4 antibodyAnti-Ncf-4 antibodyAnti-Neutrophil Nadph Oxidase Factor 4 antibodyAnti-Sh3 And Px Domain-Containing Protein 4 antibodyAnti-P40-Phox antibodyAnti-P40phox antibodyAnti-NCF4 antibodyAnti-SH3PXD4 antibody |
Information sourced from Uniprot.org