• Western blot analysis of extracts of various cell lines, using NCF4/p40-phox antibody (STJ24700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NCF4 antibody (1-190) (STJ24700)

SKU:
STJ24700

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCF4 (1-190) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCF4
Gene ID: 4689
Uniprot ID: NCF4_HUMAN
Immunogen Region: 1-190
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-190 of human NCF4/p40-phox (NP_038202.2).
Immunogen Sequence: MAVAQQLRAESDFEQLPDDV AISANIADIEEKRGFTSHFV FVIEVKTKGGSKYLIYRRYR QFHALQSKLEERFGPDSKSS ALACTLPTLPAKVYVGVKQE IAEMRIPALNAYMKSLLSLP VWVLMDEDVRIFFYQSPYDS EQVPQALRRLRPRTRKVKSV SPQGNSVDRMAAPRAEALFD FTGNSKLELN
Tissue Specificity Expression is restricted to hematopoietic cells.
Function Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Protein Name Neutrophil Cytosol Factor 4
Ncf-4
Neutrophil Nadph Oxidase Factor 4
Sh3 And Px Domain-Containing Protein 4
P40-Phox
P40phox
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1236973
Reactome: R-HSA-3299685
Reactome: R-HSA-4420097
Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Cellular Localisation Cytoplasm
Cytosol
Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Membrane
Alternative Antibody Names Anti-Neutrophil Cytosol Factor 4 antibody
Anti-Ncf-4 antibody
Anti-Neutrophil Nadph Oxidase Factor 4 antibody
Anti-Sh3 And Px Domain-Containing Protein 4 antibody
Anti-P40-Phox antibody
Anti-P40phox antibody
Anti-NCF4 antibody
Anti-SH3PXD4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance