• Immunohistochemistry analysis of paraffin-embedded mouse heart using NAT8B antibody (STJ29283) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using NAT8B antibody (STJ29283) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using NAT8B antibody (STJ29283) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of rat brain, using NAT8B antibody (STJ29283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NAT8B antibody (75-165) (STJ29283)

SKU:
STJ29283

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NAT8B (75-165) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NAT8BUniprot ID=NAT8B_HUMAN"
Immunogen Region: 75-165
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 75-165 of human NAT8B (NP_057431.2).
Immunogen Sequence: WFLAKKPWTRYVDIALRTDM SDITKSYLSECGSCFWVGES EEKVVGTVGALPVDDPTLRE KRLQLFHLSVDNEHRGQGIA KALVRTVLQFA
Function Lysine N-acetyltransferase catalyzing peptidyl-lysine N6-acetylation of various proteins, including PROM1/CD133. Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1. Acetylates and stabilizes BACE1 immature protein, leading to increased steady-state levels in neurons. By acting on BACE1 expression, may regulate amyloid beta-peptide formation. May play a role in regulation of gastrulation.
Protein Name Putative N-Acetyltransferase 8b
Acetyltransferase 1
Atase1
Camello-Like Protein 2
Cellular Localisation Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Single-Pass Type Ii Membrane Protein
Endoplasmic Reticulum Membrane
Enriched In The Endoplasmic Reticulum-Golgi Intermediate Compartment (Ergic)
Alternative Antibody Names Anti-Putative N-Acetyltransferase 8b antibody
Anti-Acetyltransferase 1 antibody
Anti-Atase1 antibody
Anti-Camello-Like Protein 2 antibody
Anti-NAT8B antibody
Anti-CML2 antibody

Information sourced from Uniprot.org

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