• Immunofluorescence analysis of paraffin-embedded rat heart using Myosin heavy chain Rabbit monoclonal antibody (STJ11102207) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded mouse heart using Myosin heavy chain Rabbit monoclonal antibody (STJ11102207) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Myosin heavy chain in paraffin-embedded mouse heart using Myosin heavy chain Rabbit monoclonal antibody (STJ11102207) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Myosin heavy chain Rabbit monoclonal antibody (STJ11102207) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MYH7 antibody (1200-1400) [S7MR] (STJ11102207)

SKU:
STJ11102207

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Myosin heavy chain (1200-1400) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1200-1400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1400 of human Myosin heavy chain (P12883).
Immunogen Sequence: VAELGEQIDNLQRVKQKLEK EKSEFKLELDDVTSNMEQII KAKANLEKMCRTLEDQMNEH RSKAEETQRSVNDLTSQRAK LQTENGELSRQLDEKEALIS QLTRGKLTYTQQLEDLKRQL EEEVKAKNALAHALQSARHD CDLLREQYEEETEAKAELQR VLSKANSEVAQWRTKYETDA IQRTEELEEAKKKLAQRLQE A
Background The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It is highly similar to the FK506-binding protein 1A. Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. There are two alternatively spliced transcript variants of this gene encoding different isoforms.

Information sourced from Uniprot.org

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