• Immunofluorescence analysis of paraffin-embedded rat heart using Myosin heavy chain Rabbit monoclonal antibody (STJ11102207) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded mouse heart using Myosin heavy chain Rabbit monoclonal antibody (STJ11102207) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Myosin heavy chain in paraffin-embedded mouse heart using Myosin heavy chain Rabbit monoclonal antibody (STJ11102207) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Myosin heavy chain Rabbit monoclonal antibody (STJ11102207) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MYH7 antibody (1200-1400) [S7MR] (STJ11102207)

SKU:
STJ11102207

$197.78 - $867.38
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:100-1:500
IF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYH6
MYH7
Gene ID: 4624
4625
Uniprot ID: MYH6_HUMAN
MYH7_HUMAN
Immunogen Region: 1200-1400
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1400 of human Myosin heavy chain (P12883).
Immunogen Sequence: VAELGEQIDNLQRVKQKLEK EKSEFKLELDDVTSNMEQII KAKANLEKMCRTLEDQMNEH RSKAEETQRSVNDLTSQRAK LQTENGELSRQLDEKEALIS QLTRGKLTYTQQLEDLKRQL EEEVKAKNALAHALQSARHD CDLLREQYEEETEAKAELQR VLSKANSEVAQWRTKYETDA IQRTEELEEAKKKLAQRLQE A
Tissue Specificity P13533 - NA
P12883 - Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level).
Alternative Antibody Names Anti-Myosin-6 antibody
Anti-Myosin Heavy Chain 6 antibody
Anti-Myosin Heavy Chain - Cardiac Muscle Alpha Isoform antibody
Anti-Myhc-Alpha antibody
Anti-MYH6 antibody
Anti-MYHCA antibody
Anti-Myosin-7 antibody
Anti-Myosin Heavy Chain 7 antibody
Anti-Myosin Heavy Chain Slow Isoform antibody
Anti-Myhc-Slow antibody
Anti-Myosin Heavy Chain - Cardiac Muscle Beta Isoform antibody
Anti-Myhc-Beta antibody
Anti-MYH7 antibody
Anti-MYHCB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance