• STJ71215 (0. 03µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-MYLK antibody (Internal) (STJ71215)

SKU:
STJ71215-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-MYLK (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.01-0.03µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MYLK
Gene ID: 4638
Uniprot ID: MYLK_HUMAN
Immunogen Region: Internal
Accession Number: NP_444253.3; NP_444254.3 ; NP_444255.3; NP_444256.3
Specificity: This antibody is expected to recognise all reported isoforms (NP_444253.3; NP_444254.3 ; NP_444255.3 and NP_444256.3).
Immunogen Sequence: KDTKNMEAKKLSKD
Post Translational Modifications Can probably be down-regulated by phosphorylation. Tyrosine phosphorylation by ABL1 increases kinase activity, reverses MLCK-mediated inhibition of Arp2/3-mediated actin polymerization, and enhances CTTN-binding. Phosphorylation by SRC at Tyr-464 and Tyr-471 promotes CTTN binding. The C-terminus is deglutamylated by AGTPBP1/CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown. Acetylated at Lys-608 by NAA10/ARD1 via a calcium-dependent signaling.this acetylation represses kinase activity and reduces tumor cell migration.
Function Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury.triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells through anti-apoptosis.
Protein Name Myosin Light Chain Kinase - Smooth Muscle
Mlck
Smmlck
Kinase-Related Protein
Krp
Telokin Cleaved Into - Myosin Light Chain Kinase - Smooth Muscle - Deglutamylated Form
Database Links Reactome: R-HSA-445355
Reactome: R-HSA-5627123
Cellular Localisation Cytoplasm
Cell Projection
Lamellipodium
Cleavage Furrow
Cytoskeleton
Stress Fiber
Localized To Stress Fibers During Interphase And To The Cleavage Furrow During Mitosis
Alternative Antibody Names Anti-Myosin Light Chain Kinase - Smooth Muscle antibody
Anti-Mlck antibody
Anti-Smmlck antibody
Anti-Kinase-Related Protein antibody
Anti-Krp antibody
Anti-Telokin Cleaved Into - Myosin Light Chain Kinase - Smooth Muscle - Deglutamylated Form antibody
Anti-MYLK antibody
Anti-MLCK antibody
Anti-MLCK1 antibody
Anti-MYLK1 antibody

Information sourced from Uniprot.org

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