• Immunofluorescence analysis of paraffin-embedded rat skeletal muscle using MYH4 Rabbit polyclonal antibody (STJ117488) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MYH4 in paraffin-embedded rat skeletal muscle using MYH4 Rabbit polyclonal antibody (STJ117488) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MYH4 in paraffin-embedded rat heart using MYH4 Rabbit polyclonal antibody (STJ117488) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MYH4 in paraffin-embedded mouse skeletal muscle using MYH4 Rabbit polyclonal antibody (STJ117488) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MYH4 in paraffin-embedded mouse heart using MYH4 Rabbit polyclonal antibody (STJ117488) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using MYH4 Rabbit polyclonal antibody (STJ117488) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of lysates from Mouse skeletal muscle, using MYH4 Rabbit polyclonal antibody (STJ117488) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MYH4 antibody (1500-1600) (STJ117488)

SKU:
STJ117488

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYH4 (1500-1600) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYH4
Gene ID: 4622
Uniprot ID: MYH4_HUMAN
Immunogen Region: 1500-1600
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1500-1600 of human MYH4 (NP_060003.2).
Immunogen Sequence: ETLKRENKNLQQEISDLTEQ IAEGGKHIHELEKVKKQLDH EKSELQTSLEEAEASLEHEE GKILRIQLELNQVKSEIDRK IAEKDEELDQLKRNHLRVVE S
Function Muscle contraction.
Protein Name Myosin-4
Myosin Heavy Chain 2b
Myhc-2b
Myosin Heavy Chain 4
Myosin Heavy Chain Iib
Myhc-Iib
Myosin Heavy Chain - Skeletal Muscle - Fetal
Cellular Localisation Cytoplasm
Myofibril
Thick Filaments Of The Myofibrils
Alternative Antibody Names Anti-Myosin-4 antibody
Anti-Myosin Heavy Chain 2b antibody
Anti-Myhc-2b antibody
Anti-Myosin Heavy Chain 4 antibody
Anti-Myosin Heavy Chain Iib antibody
Anti-Myhc-Iib antibody
Anti-Myosin Heavy Chain - Skeletal Muscle - Fetal antibody
Anti-MYH4 antibody

Information sourced from Uniprot.org

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