• Immunofluorescence analysis of rat brain using N-Myc/MYCN Rabbit polyclonal antibody (STJ24658) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain using N-Myc/MYCN Rabbit polyclonal antibody (STJ24658) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using N-Myc/MYCN antibody (STJ24658) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using N-Myc/MYCN antibody (STJ24658) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of HeLa cells, using N-Myc/MYCN antibody (STJ24658) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MYCN antibody (168-267) (STJ24658)

SKU:
STJ24658

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYCN (168-267) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYCN
Gene ID: 4613
Uniprot ID: MYCN_HUMAN
Immunogen Region: 168-267
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 168-267 of human N-Myc/MYCN (NP_005369.2).
Immunogen Sequence: AGRAGAALPAELAHPAAECV DPAVVFPFPVNKREPAPVPA APASAPAAGPAVASGAGIAA PAGAPGVAPPRPGGRQTSGG DHKALSTSGEDTLSDSDDED
Tissue Specificity Expressed in the neuronal cells of the cerebrum, neuroblastomas and thyroid tumors (at protein level).
Post Translational Modifications Phosphorylated by GSK3-beta which may promote its degradation. Phosphorylated by AURKA.
Function Positively regulates the transcription of MYCNOS in neuroblastoma cells.
Protein Name N-Myc Proto-Oncogene Protein
Class E Basic Helix-Loop-Helix Protein 37
Bhlhe37
Database Links Reactome: R-HSA-201556
Cellular Localisation Nucleus
Alternative Antibody Names Anti-N-Myc Proto-Oncogene Protein antibody
Anti-Class E Basic Helix-Loop-Helix Protein 37 antibody
Anti-Bhlhe37 antibody
Anti-MYCN antibody
Anti-BHLHE37 antibody
Anti-NMYC antibody

Information sourced from Uniprot.org

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