• Immunohistochemistry analysis of paraffin-embedded mouse heart using MYBPC3 antibody (STJ114230) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using MYBPC3 antibody (STJ114230) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of rat heart, using MYBPC3 antibody (STJ114230) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MYBPC3 antibody (200-400) (STJ114230)

SKU:
STJ114230

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYBPC3 (200-400) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYBPC3
Gene ID: 4607
Uniprot ID: MYPC3_HUMAN
Immunogen Region: 200-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human MYBPC3 (NP_000247.2).
Immunogen Sequence: SSKVGQHLQLHDSYDRASKV YLFELHITDAQPAFTGSYRC EVSTKDKFDCSNFNLTVHEA MGTGDLDLLSAFRRTSLAGG GRRISDSHEDTGILDFSSLL KKRDSFRTPRDSKLEAPAEE DVWEILRQAPPSEYERIAFQ YGVTDLRGMLKRLKGMRRDE KKSTAFQKKLEPAYQVSKGH KIRLTVELADHDAEVKWLKN G
Post Translational Modifications Substrate for phosphorylation by PKA and PKC. Reversible phosphorylation appears to modulate contraction. Polyubiquitinated.
Function Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
Protein Name Myosin-Binding Protein C - Cardiac-Type
Cardiac Mybp-C
C-Protein - Cardiac Muscle Isoform
Database Links Reactome: R-HSA-390522
Alternative Antibody Names Anti-Myosin-Binding Protein C - Cardiac-Type antibody
Anti-Cardiac Mybp-C antibody
Anti-C-Protein - Cardiac Muscle Isoform antibody
Anti-MYBPC3 antibody

Information sourced from Uniprot.org

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