• Confocal immunofluorescence analysis of U2OS cells using MYBBP1A Rabbit polyclonal antibody (STJ24653) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MYBBP1A in paraffin-embedded rat spleen using MYBBP1A Rabbit polyclonal antibody (STJ24653) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MYBBP1A in paraffin-embedded rat lung using MYBBP1A Rabbit polyclonal antibody (STJ24653) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MYBBP1A Rabbit polyclonal antibody (STJ24653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MYBBP1A antibody (1104-1328) (STJ24653)

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STJ24653

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYBBP1A (1104-1328) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYBBP1A
Gene ID: 10514
Uniprot ID: MBB1A_HUMAN
Immunogen Region: 1104-1328
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1104-1328 of human MYBBP1A (NP_055335.2).
Immunogen Sequence: DLTVLLGVLQGQQQSLQQGA HSTGSSRLHDLYWQAMKTLG VQRPKLEKKDAKEIPSATQS PISKKRKKKGFLPETKKRKK RKSEDGTPAEDGTPAATGGS QPPSMGRKKRNRTKAKVPAQ ANGTPTTKSPAPGAPTRSPS TPAKSPKLQKKNQKPSQVNG APGSPTEPAGQKQHQKALPK KGVLGKSPLSALARKKARLS LVIRSPSLLQSGAKKKAQVR KAGKP
Post Translational Modifications Citrullinated by PADI4.
Function May activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity). Acts as a corepressor and in concert with CRY1, represses the transcription of the core circadian clock component PER2. Preferentially binds to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter. Has a role in rRNA biogenesis together with PWP1.
Protein Name Myb-Binding Protein 1a
Database Links Reactome: R-HSA-5250924
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Shuttles Between The Nucleus And Cytoplasm
Nuclear Import May Be Mediated By Kpna2
While Export Appears To Depend Partially On Xpo1/Crm1
Predominantly Nucleolar
Alternative Antibody Names Anti-Myb-Binding Protein 1a antibody
Anti-MYBBP1A antibody
Anti-P160 antibody

Information sourced from Uniprot.org

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