• Immunofluorescence analysis of U-2 OS cells using MTMR4 Rabbit polyclonal antibody (STJ117544) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using MTMR4 Rabbit polyclonal antibody (STJ117544) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using MTMR4 Rabbit polyclonal antibody (STJ117544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MTMR4 antibody (920-1195) (STJ117544)

SKU:
STJ117544

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTMR4 (920-1195) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTMR4
Gene ID: 9110
Uniprot ID: MTMR4_HUMAN
Immunogen Region: 920-1195
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 920-1195 of human MTMR4 (NP_004678.3).
Immunogen Sequence: GEATPRRLLSYGCCSKRPNS KQMRATGPCFGGQWAQREGV KSPVCSSHSNGHCTGPGGKN QMWLSSHPKQVSSTKPVPLN CPSPVPPLYLDDDGLPFPTD VIQHRLRQIEAGYKQEVEQL RRQVRELQMRLDIRHCCAPP AEPPMDYEDDFTCLKESDGS DTEDFGSDHSEDCLSEASWE PVDKKETEVTRWVPDHMASH CYNCDCEFWLAKRRHHCRNC GNVFCAGCCHLKLPIPDQQ
Tissue Specificity Expressed in brain, heart, kidney, spleen, liver, colon, testis, muscle, placenta, thyroid gland, pancreas, ovary, prostate, skin, peripheral blood, and bone marrow.
Function Dephosphorylates proteins phosphorylated on Ser, Thr, and Tyr residues and low molecular weight phosphatase substrate para-nitrophenylphosphate. Phosphorylates phosphatidylinositol 3,4,5-trisphosphate (PIP3).
Protein Name Myotubularin-Related Protein 4
Fyve Domain-Containing Dual Specificity Protein Phosphatase 2
Fyve-Dsp2
Zinc Finger Fyve Domain-Containing Protein 11
Database Links Reactome: R-HSA-1660516
Reactome: R-HSA-1660517
Reactome: R-HSA-2173788
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Localized To Perinuclear Region
Alternative Antibody Names Anti-Myotubularin-Related Protein 4 antibody
Anti-Fyve Domain-Containing Dual Specificity Protein Phosphatase 2 antibody
Anti-Fyve-Dsp2 antibody
Anti-Zinc Finger Fyve Domain-Containing Protein 11 antibody
Anti-MTMR4 antibody
Anti-KIAA0647 antibody
Anti-ZFYVE11 antibody

Information sourced from Uniprot.org

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