• Western blot analysis of various lysates, using MTHFD2 Rabbit monoclonal antibody (STJ11104901) at 1:7000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MTHFD2 antibody (40-250) [S4901RM] (STJ11104901)

SKU:
STJ11104901

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Bifunctional Methylenetetrahydrofolate Dehydrogenase/Cyclohydrolase-Mitochondrial Includes-Nad-Dependent Methylenetetrahydrofolate Dehydrogenase-Methenyltetrahydrofolate Cyclohydrolase (40-250) is suitable for use in W
Clonality: Monoclonal
Clone ID: S4901RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:8000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTHFD2
Gene ID: 10797
Uniprot ID: MTDC_HUMAN
Immunogen Region: 40-250
Immunogen: Recombinant funsion protein containing a sequence corresponding to amino acids 40-250 of human MTHFD2 (NP_006627.2).
Immunogen Sequence: ISGRKLAQQIKQEVRQEVEE WVASGNKRPHLSVILVGENP ASHSYVLNKTRAAAVVGINS ETIMKPASISEEELLNLINK LNNDDNVDGLLVQLPLPEHI DERRICNAVSPDKDVDGFHV INVGRMCLDQYSMLPATPWG VWEIIKRTGIPTLGKNVVVA GRSKNVGMPIAMLLHTDGAH ERPGGDATVTISHRYTPKEQ LKKHTILADIV
Function Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
Protein Name Bifunctional Methylenetetrahydrofolate Dehydrogenase/Cyclohydrolase - Mitochondrial Includes - Nad-Dependent Methylenetetrahydrofolate Dehydrogenase - Methenyltetrahydrofolate Cyclohydrolase
Database Links Reactome: R-HSA-196757
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Bifunctional Methylenetetrahydrofolate Dehydrogenase/Cyclohydrolase - Mitochondrial Includes - Nad-Dependent Methylenetetrahydrofolate Dehydrogenase - Methenyltetrahydrofolate Cyclohydrolase antibody
Anti-MTHFD2 antibody
Anti-NMDMC antibody

Information sourced from Uniprot.org

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