• Western blot analysis of various lysates, using GDF-8/Myostatin Rabbit monoclonal antibody (STJ11104931) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-MSTN antibody (24-375) [S4931RM] (STJ11104931)

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STJ11104931

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Growth/Differentiation Factor 8 (24-375) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4931RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSTN
Gene ID: 2660
Uniprot ID: GDF8_HUMAN
Immunogen Region: 24-375
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-375 of human GDF-8/Myostatin (NP_005250.1).
Immunogen Sequence: NENSEQKENVEKEGLCNACT WRQNTKSSRIEAIKIQILSK LRLETAPNISKDVIRQLLPK APPLRELIDQYDVQRDDSSD GSLEDDDYHATTETIITMPT ESDFLMQVDGKPKCCFFKFS SKIQYNKVVKAQLWIYLRPV ETPTTVFVQILRLIKPMKDG TRYTGIRSLKLDMNPGTGIW QSIDVKTVLQNWLKQPESNL GIEIKALDENGHDLAVTFPG PGEDGLNPFLEVKVTDTPK
Post Translational Modifications Synthesized as large precursor molecule that undergoes proteolytic cleavage to generate an N-terminal propeptide and a disulfide linked C-terminal dimer, which is the biologically active molecule. The circulating form consists of a latent complex of the C-terminal dimer and other proteins, including its propeptide, which maintain the C-terminal dimer in a latent, inactive state. Ligand activation requires additional cleavage of the prodomain by a tolloid-like metalloproteinase.
Function Acts specifically as a negative regulator of skeletal muscle growth.
Protein Name Growth/Differentiation Factor 8
Gdf-8
Myostatin
Database Links Reactome: R-HSA-9617828
Cellular Localisation Secreted
Alternative Antibody Names Anti-Growth/Differentiation Factor 8 antibody
Anti-Gdf-8 antibody
Anti-Myostatin antibody
Anti-MSTN antibody
Anti-GDF8 antibody

Information sourced from Uniprot.org

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