Immunohistochemistry analysis ofย MRPS36 in paraffin-embeddedย human colon tissue usingย MRPS36 Rabbit polyclonal antibody (STJ11105449) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-MRPS36 antibody (1-103) (STJ11105449)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11105449
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General Information

Short DescriptionRabbit polyclonal Alpha-Ketoglutarate Dehydrogenase Component 4 (1-103) antibody for use in WB, IHC-P and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolKGD4
Gene ID92259
Uniprot IDKGD4_HUMAN
Immunogen Region1-103
Immunogen SequenceMMGSKMASASRVVQVVKPHT PLIRFPDRRDNPKPNVSEAL RSAGLPSHSSVISQHSKGSK SPDLLMYQGPPDTAEIIKTL PQKYRRKLVSQEEMEFIQRG GPE
SpecificityRecombinant Protein corresponding to a sequence within amino acids 1-103 of human MRPS36 (NP_150597.1).

Additional Info

Function Molecular adapter that is necessary to form a stable 2-oxoglutarate dehydrogenase enzyme complex (OGDHC). Enables the specific recruitment of E3 subunit to E2 subunit in the 2-oxoglutarate dehydrogenase complex (OGDHC).
Protein Name Alpha-Ketoglutarate Dehydrogenase Component 4
Alpha-Ketoglutarate Dehydrogenase Subunit 4
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Reactome: R-HSA-6783984
Reactome: R-HSA-9853506
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Alpha-Ketoglutarate Dehydrogenase Component 4 antibody
Anti-Alpha-Ketoglutarate Dehydrogenase Subunit 4 antibody
Anti-KGD4 antibody
Anti-MRPS36 antibody
Anti-DC47 antibody

Information sourced from Uniprot.org

Citations

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