• Immunohistochemistry analysis of MRPS36 in paraffin-embedded human colon tissue using MRPS36 Rabbit polyclonal antibody (STJ11105449) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from Rat heart using MRPS36 Rabbit polyclonal antibody (STJ11105449) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:5s.
  • Western blot analysis of lysates from A549 cells using MRPS36 Rabbit polyclonal antibody (STJ11105449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MRPS36 antibody (1-103) (STJ11105449)

SKU:
STJ11105449

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Alpha-Ketoglutarate Dehydrogenase Component 4 (1-103) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPS36
Gene ID: 92259
Uniprot ID: KGD4_HUMAN
Immunogen Region: 1-103
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 1-103 of human MRPS36 (NP_150597.1).
Immunogen Sequence: MMGSKMASASRVVQVVKPHT PLIRFPDRRDNPKPNVSEAL RSAGLPSHSSVISQHSKGSK SPDLLMYQGPPDTAEIIKTL PQKYRRKLVSQEEMEFIQRG GPE
Function May be necessary to recruit DLD/E3 to the mitochondrial 2-oxoglutarate dehydrogenase complex (OGDC) core composed of OGDH/E1-DLST/E2, hence stabilizes the complex.
Protein Name Alpha-Ketoglutarate Dehydrogenase Component 4
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Alpha-Ketoglutarate Dehydrogenase Component 4 antibody
Anti-MRPS36 antibody
Anti-KGD4 antibody
Anti-DC47 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance