| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MRPS27 (255-414) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MRPS27 |
| Gene ID: |
23107 |
| Uniprot ID: |
RT27_HUMAN |
| Immunogen Region: |
255-414 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 255-414 of human MRPS27 (NP_055899.2). |
| Immunogen Sequence: |
YLDRALQVMEKVAASPEDIK LCREALDVLGAVLKALTSAD GASEEQSQNDEDNQGSEKLV EQLDIEETEQSKLPQYLERF KALHSKLQALGKIESEGLLS LTTQLVKEKLSTCEAEDIAT YEQNLQQWHLDLVQLIQREQ QQREQAKQEYQAQKAAKASA |
| Tissue Specificity | Overexpressed in hepatocellular carcinoma tissues compared with adjacent non-tumoral liver tissues (at protein level). Ubiquitous. |
| Function | RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis. Stimulates mitochondrial mRNA translation of subunit components of the mitochondrial electron transport chain. Binds to the mitochondrial 12S rRNA (12S mt-rRNA) and tRNA(Glu). Involved also in positive regulation of cell proliferation and tumor cell growth. |
| Protein Name | Small Ribosomal Subunit Protein Ms2728s Ribosomal Protein S27 - MitochondrialMrp-S27S27mtMitochondrial Ribosomal Protein S27 |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276 |
| Cellular Localisation | CytoplasmMitochondrion |
| Alternative Antibody Names | Anti-Small Ribosomal Subunit Protein Ms27 antibodyAnti-28s Ribosomal Protein S27 - Mitochondrial antibodyAnti-Mrp-S27 antibodyAnti-S27mt antibodyAnti-Mitochondrial Ribosomal Protein S27 antibodyAnti-MRPS27 antibodyAnti-KIAA0264 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and IDH1 knockout (KO) HeLa cells, using [KO Validated] IDH1 Rabbit polyclonal antibody (STJ119996) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98781/382830/STJ119996_1__99034.1713151333.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IDH1 knockout (KO) HeLa cells, using [KO Validated] IDH1 Rabbit polyclonal antibody (STJ119996) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
