![Western blot analysis of lysates from U-251 MG cells using [KD Validated] MRPL52 Rabbit polyclonal antibody (STJ11105663) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:45s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178305/370696/STJ11105663_1__68920.1713136634.jpg?c=1 "Western blot analysis of lysates from U-251 MG cells using [KD Validated] MRPL52 Rabbit polyclonal antibody (STJ11105663) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:45s.")
![Western blot analysis of lysates from wild type (WT) and MRPL52 knockdown (KD) HeLa cells using [KD Validated] MRPL52 Rabbit polyclonal antibody (STJ11105663) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 45s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178305/370697/STJ11105663_2__64160.1713136634.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MRPL52 knockdown (KD) HeLa cells using [KD Validated] MRPL52 Rabbit polyclonal antibody (STJ11105663) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 45s.")
![Western blot analysis of lysates from U-251 MG cells using [KD Validated] MRPL52 Rabbit polyclonal antibody (STJ11105663) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:45s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/178305/370696/STJ11105663_1__68920.1713136634.jpg?c=1 "Western blot analysis of lysates from U-251 MG cells using [KD Validated] MRPL52 Rabbit polyclonal antibody (STJ11105663) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:45s.")
Anti-MRPL52 antibody (24-123) (STJ11105663)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Large Ribosomal Subunit Protein Ml52 (24-123) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MRPL52 |
| Gene ID | 122704 |
| Uniprot ID | RM52_HUMAN |
| Immunogen Region | 24-123 |
| Immunogen Sequence | GGQWRLQQGLAANPSGYGPL TELPDWSYADGRPAPPMKGQ LRRKAERETFARRVVLLSQE MDAGLQAWQLRQQKLQEEQR KQENALKPKGASLKSPLPSQ |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 24-123 of human MRPL52 (NP_848026.1). |
Additional Info
| Protein Name | Large Ribosomal Subunit Protein Ml5239s Ribosomal Protein L52 - MitochondrialL52mtMrp-L52 |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Ml52 antibodyAnti-39s Ribosomal Protein L52 - Mitochondrial antibodyAnti-L52mt antibodyAnti-Mrp-L52 antibodyAnti-MRPL52 antibody |
Information sourced from Uniprot.org