| Short Description : | Rabbit polyclonal MRPL27 (49-148 aa) antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MRPL27 |
| Gene ID: | 51264 |
| Uniprot ID: | RM27_HUMAN |
| Immunogen Region: | 49-148 aa |
| Immunogen Sequence: | RRQGIKKMEGHYVHAGNIIA TQRHFRWHPGAHVGVGKNKC LYALEEGIVRYTKEVYVPHP RNTEAVDLITRLPKGAVLYK TFVHVVPAKPEGTFKLVAML |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 49-148 of human MRPL27 (NP_057588.1). |
| Protein Name | Large Ribosomal Subunit Protein Bl27m39s Ribosomal Protein L27 - MitochondrialL27mtMrp-L27 |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Bl27m antibodyAnti-39s Ribosomal Protein L27 - Mitochondrial antibodyAnti-L27mt antibodyAnti-Mrp-L27 antibodyAnti-MRPL27 antibodyAnti-HSPC250 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MRPL27 knockdown (KD) 293T cells using [KD Validated] MRPL27 Rabbit pAb (STJ11107034) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186230/407914/STJ11107034_1__15921.1744894382.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MRPL27 knockdown (KD) 293T cells using [KD Validated] MRPL27 Rabbit pAb (STJ11107034) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.")
![Immunohistochemistry analysis of paraffin-embedded Human cervical squamous cell carcinoma tissue using [KD Validated] MRPL27 Rabbit pAb (STJ11107034) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186230/407915/STJ11107034_2__63700.1744894382.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Human cervical squamous cell carcinoma tissue using [KD Validated] MRPL27 Rabbit pAb (STJ11107034) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] MRPL27 Rabbit pAb (STJ11107034) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186230/407916/STJ11107034_3__69771.1744894383.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] MRPL27 Rabbit pAb (STJ11107034) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Western blot analysis of lysates from wild type (WT) and MRPL27 knockdown (KD) 293T cells using [KD Validated] MRPL27 Rabbit pAb (STJ11107034) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/186230/407914/STJ11107034_1__15921.1744894382.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MRPL27 knockdown (KD) 293T cells using [KD Validated] MRPL27 Rabbit pAb (STJ11107034) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.")
