Anti-MRPL12 antibody (1-198) (STJ110616)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MRPL12 (1-198) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MRPL12 |
| Gene ID | 6182 |
| Uniprot ID | RM12_HUMAN |
| Immunogen Region | 1-198 |
| Immunogen Sequence | MLPAAARPLWGPCLGLRAAA FRLARRQVPCVCAVRHMRSS GHQRCEALAGAPLDNAPKEY PPKIQQLVQDIASLTLLEIS DLNELLKKTLKIQDVGLVPM GGVMSGAVPAAAAQEAVEED IPIAKERTHFTVRLTEAKPV DKVKLIKEIKNYIQGINLVQ AKKLVESLPQEIKANVAKAE AEKIKAALEAVGGTVVLE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human MRPL12 (NP_002940.2). |
Additional Info
| Post Translational Modifications | Two mature forms are produced by differential two-step proteolytic cleavage. Cleaved by the mitochondrial processing protease to produce the long mature form and subsequently by the mitochondrial intermediate protease to produce the short mature form. In the presence of CUL3, undergoes 'Lys-63'-linked ubiquitination at Lys-150 which results in proteasomal degradation. |
| Function | As a component of the mitochondrial large ribosomal subunit, plays a role in mitochondrial translation. When present in mitochondria as a free protein not associated with the ribosome, associates with mitochondrial RNA polymerase POLRMT to activate transcription. Required for POLRMT stability. |
| Protein Name | Large Ribosomal Subunit Protein Bl12m39s Ribosomal Protein L12 - MitochondrialL12mtMrp-L125c5-2 Cleaved Into - Large Ribosomal Subunit Protein Bl12m - Long Mature Form - Large Ribosomal Subunit Protein Bl12m - Short Mature Form |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Bl12m antibodyAnti-39s Ribosomal Protein L12 - Mitochondrial antibodyAnti-L12mt antibodyAnti-Mrp-L12 antibodyAnti-5c5-2 Cleaved Into - Large Ribosomal Subunit Protein Bl12m - Long Mature Form - Large Ribosomal Subunit Protein Bl12m - Short Mature Form antibodyAnti-MRPL12 antibodyAnti-MRPL7 antibodyAnti-RPML12 antibody |
Information sourced from Uniprot.org