• CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of H3K18Me1 in representative gene loci (MYT1) , as shown in figure.
  • Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K18 antibody (STJ11103411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Dot-blot analysis of all sorts of peptides using MonoMethyl-Histone H3-K18 antibody (STJ11103411) at 1:1000 dilution.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 600 Mu g extracts of 293F cells using 5 Mu g MonoMethyl-Histone H3-K18 antibody (STJ11103411). Western blot was performed from the immunoprecipitate using MonoMethyl-Histone H3-K18 antibody (STJ11103411) at a dilution of 1:1000.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K18 antibody (STJ11103411) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Mono-Methyl-Histone H3-K18 antibody [S1MR] (STJ11103411)

SKU:
STJ11103411

£146.50 - £642.50
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Host: Rabbit
Applications: WB/DB/IHC-P/IF/ICC/IP/ELISA/ChIP/CUT&Tag
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
DB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H3-4
H3C1.H3C2.H3C3.H3C4.H3C6.
Gene ID: 8290
8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
Uniprot ID: H31T_HUMAN
H31_HUMAN
Specificity: A synthetic monomethylated peptide around K18 of human histone H3 (P68431).
Immunogen Sequence: APRKQ
Tissue Specificity
Alternative Antibody Names Anti-Histone H3.1t antibody
Anti-H3/T antibody
Anti-H3t antibody
Anti-H3/G antibody
Anti-Histone H3.4 antibody
Anti-H3-4 antibody
Anti-H3FT antibody
Anti-HIST3H3 antibody
Anti-Histone H3.1 antibody
Anti-Histone H3/A antibody
Anti-Histone H3/B antibody
Anti-Histone H3/C antibody
Anti-Histone H3/D antibody
Anti-Histone H3/F antibody
Anti-Histone H3/H antibody
Anti-Histone H3/I antibody
Anti-Histone H3/J antibody
Anti-Histone H3/K antibody
Anti-Histone H3/L antibody
Anti-H3C1 antibody
Anti-H3FA antibody
Anti-HIST1H3A.H3C2 antibody
Anti-H3FL antibody
Anti-HIST1H3B.H3C3 antibody
Anti-H3FC antibody
Anti-HIST1H3C.H3C4 antibody
Anti-H3FB antibody
Anti-HIST1H3D.H3C6 antibody
Anti-H3FD antibody
Anti-HIST1H3E.H3C7 antibody
Anti-H3FI antibody
Anti-HIST1H3F.H3C8 antibody
Anti-H3FH antibody
Anti-HIST1H3G.H3C10 antibody
Anti-H3FK antibody
Anti-HIST1H3H.H3C11 antibody
Anti-H3FF antibody
Anti-HIST1H3I.H3C12 antibody
Anti-H3FJ antibody
Anti-HIST1H3J antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance