Anti-MOCS3 antibody (271-460) (STJ29505)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MOCS3 (271-460) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MOCS3 |
| Gene ID | 27304 |
| Uniprot ID | MOCS3_HUMAN |
| Immunogen Region | 271-460 |
| Immunogen Sequence | YSGSLLLFDALRGHFRSIRL RSRRLDCAACGERPTVTDLL DYEAFCGSSATDKCRSLQLL SPEERVSVTDYKRLLDSGAF HLLLDVRPQVEVDICRLPHA LHIPLKHLERRDAESLKLLK EAIWEEKQGTQEGAAVPIYV ICKLGNDSQKAVKILQSLSA AQELDPLTVRDVVGGLMAWA AKIDGTFPQY |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 271-460 of human MOCS3 (NP_055299.1). |
Additional Info
| Function | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor.NFS1 acting as a sulfur donor for thiocarboxylation reactions. |
| Protein Name | Adenylyltransferase And Sulfurtransferase Mocs3Molybdenum Cofactor Synthesis Protein 3Molybdopterin Synthase SulfurylaseMpt Synthase Sulfurylase Includes - Molybdopterin-Synthase AdenylyltransferaseAdenylyltransferase Mocs3Sulfur Carrier Protein Mocs2a Adenylyltransferase - Molybdopterin-Synthase SulfurtransferaseSulfur Carrier Protein Mocs2a SulfurtransferaseSulfurtransferase Mocs3 |
| Database Links | Reactome: R-HSA-947581 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Adenylyltransferase And Sulfurtransferase Mocs3 antibodyAnti-Molybdenum Cofactor Synthesis Protein 3 antibodyAnti-Molybdopterin Synthase Sulfurylase antibodyAnti-Mpt Synthase Sulfurylase Includes - Molybdopterin-Synthase Adenylyltransferase antibodyAnti-Adenylyltransferase Mocs3 antibodyAnti-Sulfur Carrier Protein Mocs2a Adenylyltransferase - Molybdopterin-Synthase Sulfurtransferase antibodyAnti-Sulfur Carrier Protein Mocs2a Sulfurtransferase antibodyAnti-Sulfurtransferase Mocs3 antibodyAnti-MOCS3 antibodyAnti-UBA4 antibody |
Information sourced from Uniprot.org