• Western blot analysis of extracts of various cell lines, using MOCS3 antibody (STJ29505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MOCS3 antibody (271-460) (STJ29505)

SKU:
STJ29505

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MOCS3 (271-460) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MOCS3
Gene ID: 27304
Uniprot ID: MOCS3_HUMAN
Immunogen Region: 271-460
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 271-460 of human MOCS3 (NP_055299.1).
Immunogen Sequence: YSGSLLLFDALRGHFRSIRL RSRRLDCAACGERPTVTDLL DYEAFCGSSATDKCRSLQLL SPEERVSVTDYKRLLDSGAF HLLLDVRPQVEVDICRLPHA LHIPLKHLERRDAESLKLLK EAIWEEKQGTQEGAAVPIYV ICKLGNDSQKAVKILQSLSA AQELDPLTVRDVVGGLMAWA AKIDGTFPQY
Function Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor.NFS1 acting as a sulfur donor for thiocarboxylation reactions.
Protein Name Adenylyltransferase And Sulfurtransferase Mocs3
Molybdenum Cofactor Synthesis Protein 3
Molybdopterin Synthase Sulfurylase
Mpt Synthase Sulfurylase Includes - Molybdopterin-Synthase Adenylyltransferase
Adenylyltransferase Mocs3
Sulfur Carrier Protein Mocs2a Adenylyltransferase - Molybdopterin-Synthase Sulfurtransferase
Sulfur Carrier Protein Mocs2a Sulfurtransferase
Sulfurtransferase Mocs3
Database Links Reactome: R-HSA-947581
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Adenylyltransferase And Sulfurtransferase Mocs3 antibody
Anti-Molybdenum Cofactor Synthesis Protein 3 antibody
Anti-Molybdopterin Synthase Sulfurylase antibody
Anti-Mpt Synthase Sulfurylase Includes - Molybdopterin-Synthase Adenylyltransferase antibody
Anti-Adenylyltransferase Mocs3 antibody
Anti-Sulfur Carrier Protein Mocs2a Adenylyltransferase - Molybdopterin-Synthase Sulfurtransferase antibody
Anti-Sulfur Carrier Protein Mocs2a Sulfurtransferase antibody
Anti-Sulfurtransferase Mocs3 antibody
Anti-MOCS3 antibody
Anti-UBA4 antibody

Information sourced from Uniprot.org

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