| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MLF1 |
| Gene ID: | 4291 |
| Uniprot ID: | MLF1_HUMAN |
| Immunogen Region: | 1-268 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human MLF1 (NP_071888.1). |
| Immunogen Sequence: | MFRMLNSSFEDDPFFSESIL AHRENMRQMIRSFSEPFGRD LLSISDGRGRAHNRRGHNDG EDSLTHTDVSSFQTMDQMVS NMRNYMQKLERNFGQLSVDP NGHSFCSSSVMTYSKIGDEP PKVFQASTQTRRAPGGIKET RKAMRDSDSGLEKMAIGHHI HDRAHVIKKSKNKKTGDEEV NQEFINMNESDAHAFDEEWQ SEVLKYKPGRHNLGNTRMRS VGHENPGSRELKRREKPQQ |
| Tissue Specificity | Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes. |
| Post Translational Modifications | Phosphorylation is required for binding to YWHAZ. |
| Function | Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus. |
| Protein Name | Myeloid Leukemia Factor 1Myelodysplasia-Myeloid Leukemia Factor 1 |
| Cellular Localisation | CytoplasmNucleusCell ProjectionCiliumCytoskeletonCilium Basal BodyShuttles Between The Cytoplasm And Nucleus |
| Alternative Antibody Names | Anti-Myeloid Leukemia Factor 1 antibodyAnti-Myelodysplasia-Myeloid Leukemia Factor 1 antibodyAnti-MLF1 antibody |
Information sourced from Uniprot.org