| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-METAP2 (1-220) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
METAP2 |
| Gene ID: |
10988 |
| Uniprot ID: |
MAP2_HUMAN |
| Immunogen Region: |
1-220 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human METAP2 (NP_006829.1). |
| Immunogen Sequence: |
MAGVEEVAASGSHLNGDLDP DDREEGAASTAEEAAKKKRR KKKKSKGPSAAGEQEPDKES GASVDEVARQLERSALEDKE RDEDDEDGDGDGDGATGKKK KKKKKKRGPKVQTDPPSVPI CDLYPNGVFPKGQECEYPPT QDGRTAAWRTTSEEKKALDQ ASEEIWNDFREAAEAHRQVR KYVMSWIKPGMTMIEICEKL EDCSRKLIKENGLNAGLAFP |
| Post Translational Modifications | Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding. |
| Function | Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. |
| Protein Name | Methionine Aminopeptidase 2Map 2Metap 2Initiation Factor 2-Associated 67 Kda GlycoproteinP67P67eif2Peptidase M |
| Database Links | Reactome: R-HSA-2514859 |
| Cellular Localisation | CytoplasmAbout 30% Of Expressed Metap2 Associates With Polysomes |
| Alternative Antibody Names | Anti-Methionine Aminopeptidase 2 antibodyAnti-Map 2 antibodyAnti-Metap 2 antibodyAnti-Initiation Factor 2-Associated 67 Kda Glycoprotein antibodyAnti-P67 antibodyAnti-P67eif2 antibodyAnti-Peptidase M antibodyAnti-METAP2 antibodyAnti-MNPEP antibodyAnti-P67EIF2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
