Anti-METAP2 antibody (1-220) (STJ111259)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal METAP2 (1-220) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | METAP2 |
| Gene ID | 10988 |
| Uniprot ID | MAP2_HUMAN |
| Immunogen Region | 1-220 |
| Immunogen Sequence | MAGVEEVAASGSHLNGDLDP DDREEGAASTAEEAAKKKRR KKKKSKGPSAAGEQEPDKES GASVDEVARQLERSALEDKE RDEDDEDGDGDGDGATGKKK KKKKKKRGPKVQTDPPSVPI CDLYPNGVFPKGQECEYPPT QDGRTAAWRTTSEEKKALDQ ASEEIWNDFREAAEAHRQVR KYVMSWIKPGMTMIEICEKL EDCSRKLIKENGLNAGLAFP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human METAP2 (NP_006829.1). |
Additional Info
| Post Translational Modifications | Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding. |
| Function | Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. |
| Protein Name | Methionine Aminopeptidase 2Map 2Metap 2Initiation Factor 2-Associated 67 Kda GlycoproteinP67P67eif2Peptidase M |
| Database Links | Reactome: R-HSA-2514859 |
| Cellular Localisation | CytoplasmAbout 30% Of Expressed Metap2 Associates With Polysomes |
| Alternative Antibody Names | Anti-Methionine Aminopeptidase 2 antibodyAnti-Map 2 antibodyAnti-Metap 2 antibodyAnti-Initiation Factor 2-Associated 67 Kda Glycoprotein antibodyAnti-P67 antibodyAnti-P67eif2 antibodyAnti-Peptidase M antibodyAnti-METAP2 antibodyAnti-MNPEP antibodyAnti-P67EIF2 antibody |
Information sourced from Uniprot.org