• Western blot analysis of extracts of various cell lines, using METAP2 antibody (STJ111259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-METAP2 antibody (1-220) (STJ111259)

SKU:
STJ111259

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-METAP2 (1-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: METAP2
Gene ID: 10988
Uniprot ID: MAP2_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human METAP2 (NP_006829.1).
Immunogen Sequence: MAGVEEVAASGSHLNGDLDP DDREEGAASTAEEAAKKKRR KKKKSKGPSAAGEQEPDKES GASVDEVARQLERSALEDKE RDEDDEDGDGDGDGATGKKK KKKKKKRGPKVQTDPPSVPI CDLYPNGVFPKGQECEYPPT QDGRTAAWRTTSEEKKALDQ ASEEIWNDFREAAEAHRQVR KYVMSWIKPGMTMIEICEKL EDCSRKLIKENGLNAGLAFP
Post Translational Modifications Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
Function Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
Protein Name Methionine Aminopeptidase 2
Map 2
Metap 2
Initiation Factor 2-Associated 67 Kda Glycoprotein
P67
P67eif2
Peptidase M
Database Links Reactome: R-HSA-2514859
Cellular Localisation Cytoplasm
About 30% Of Expressed Metap2 Associates With Polysomes
Alternative Antibody Names Anti-Methionine Aminopeptidase 2 antibody
Anti-Map 2 antibody
Anti-Metap 2 antibody
Anti-Initiation Factor 2-Associated 67 Kda Glycoprotein antibody
Anti-P67 antibody
Anti-P67eif2 antibody
Anti-Peptidase M antibody
Anti-METAP2 antibody
Anti-MNPEP antibody
Anti-P67EIF2 antibody

Information sourced from Uniprot.org

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