• Immunofluorescence analysis of U2OS cells using MEST antibody (STJ29011). Blue: DAPI for nuclear staining.
  • Western blot analysis of Mouse brain, using MEST antibody (STJ29011) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MEST antibody (146-335) (STJ29011)

SKU:
STJ29011

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MEST (146-335) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MEST
Gene ID: 4232
Uniprot ID: MEST_HUMAN
Immunogen Region: 146-335
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 146-335 of human MEST (NP_002393.2).
Immunogen Sequence: HDYGDIVAQELLYRYKQNRS GRLTIKSLCLSNGGIFPETH RPLLLQKLLKDGGVLSPILT RLMNFFVFSRGLTPVFGPYT RPSESELWDMWAGIRNNDGN LVIDSLLQYINQRKKFRRRW VGALASVTIPIHFIYGPLDP VNPYPEFLELYRKTLPRSTV SILDDHISHYPQLEDPMGFL NAYMGFINSF
Tissue Specificity Highly expressed in hydatidiform moles, but barely expressed in dermoid cysts. Biallelic expression is detected in blood lymphocytes. Seems to imprinted in an isoform-specific manner rather than in a tissue-specific manner in lymphocytes. Isoform 1 is expressed only from the paternal allele. Isoform 2 is expressed from both the paternal allele and the maternal allele.
Protein Name Mesoderm-Specific Transcript Homolog Protein
Paternally-Expressed Gene 1 Protein
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Mesoderm-Specific Transcript Homolog Protein antibody
Anti-Paternally-Expressed Gene 1 Protein antibody
Anti-MEST antibody
Anti-PEG1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance