| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MED20 (1-212) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MED20 |
| Gene ID: |
9477 |
| Uniprot ID: |
MED20_HUMAN |
| Immunogen Region: |
1-212 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human MED20 (NP_004266.2). |
| Immunogen Sequence: |
MGVTCVSQMPVAEGKSVQQT VELLTRKLEMLGAEKQGTFC VDCETYHTAASTLGSQGQTG KLMYVMHNSEYPLSCFALFE NGPCLIADTNFDVLMVKLKG FFQSAKASKIETRGTRYQYC DFLVKVGTVTMGPSARGISV EVEYGPCVVASDCWSLLLEF LQSFLGSHTPGAPAVFGNRH DAVYGPADTMVQYMELFNKI RKQQQVPVAGIR |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
| Protein Name | Mediator Of Rna Polymerase Ii Transcription Subunit 20Mediator Complex Subunit 20Trf-Proximal Protein HomologHtrfp |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-212436Reactome: R-HSA-381340 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 20 antibodyAnti-Mediator Complex Subunit 20 antibodyAnti-Trf-Proximal Protein Homolog antibodyAnti-Htrfp antibodyAnti-MED20 antibodyAnti-TRFP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PDCD10 knockout (KO) HeLa cells, using [KO Validated] PDCD10 Rabbit polyclonal antibody (STJ117595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/96554/379107/STJ117595_1__37100.1713146827.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PDCD10 knockout (KO) HeLa cells, using [KO Validated] PDCD10 Rabbit polyclonal antibody (STJ117595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.")
