| Host: | Mouse |
| Applications: | WB/IHC/ICC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-MeCP2 (Full Length) is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 5H12 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2b |
| Formulation: | 100 ul in PBS + 50% Glycerol and 5 mM Sodium Azide |
| Purification: | This antibody was protein g purified culture from supernatant. |
| Dilution Range: | WB 1:2000IHC 1:500-1:5000ICC 1:1000-1:5000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MECP2 |
| Gene ID: | 4204 |
| Uniprot ID: | MECP2_HUMAN |
| Immunogen Region: | Full Length |
| Immunogen: | Full length human recombinant MeCP2. |
| Tissue Specificity | Present in all adult somatic tissues tested. |
| Post Translational Modifications | Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). |
| Protein Name | Methyl-Cpg-Binding Protein 2Mecp-2 ProteinMecp2 |
| Database Links | Reactome: R-HSA-8986944Reactome: R-HSA-9022534Reactome: R-HSA-9022535Reactome: R-HSA-9022537Reactome: R-HSA-9022538Reactome: R-HSA-9022692Reactome: R-HSA-9022699Reactome: R-HSA-9022702Reactome: R-HSA-9022707Reactome: R-HSA-9022927Reactome: R-HSA-9725371 |
| Cellular Localisation | NucleusColocalized With Methyl-Cpg In The GenomeColocalized With Tbl1x To The Heterochromatin Foci |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Protein 2 antibodyAnti-Mecp-2 Protein antibodyAnti-Mecp2 antibodyAnti-MECP2 antibody |
Information sourced from Uniprot.org