Anti-MDFI antibody (1-160) (STJ115663)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MDFI (1-160) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MDFI |
| Gene ID | 4188 |
| Uniprot ID | MDFI_HUMAN |
| Immunogen Region | 1-160 |
| Immunogen Sequence | MYQVSGQRPSGCDAPYGAPS AAPGPAQTLSLLPGLEVVTG STHPAEAAPEEGSLEEAATP MPQGNGPGIPQGLDSTDLDV PTEAVTCQPQGNPLGCTPLL PNDSGHPSELGGTRRAGNGA LGGPKAHRKLQTHPSLASQG SKKSKSSSKSTTSQIPLQAQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MDFI (NP_005577.1). |
Additional Info
| Function | Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. Regulates the activity of mechanosensitive Piezo channel. |
| Protein Name | Myod Family InhibitorMyogenic Repressor I-Mf |
| Database Links | |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Myod Family Inhibitor antibodyAnti-Myogenic Repressor I-Mf antibodyAnti-MDFI antibody |
Information sourced from Uniprot.org