Anti-MCM3 antibody (641-690 aa) (STJ96582)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human MCM3 at the amino acid range 641-690
General Information
| Short Description | Rabbit polyclonal anti-DNA replication licensing factor MCM3 (641-690 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC-P 1:100-300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MCM3 |
| Gene ID | 4172 |
| Uniprot ID | MCM3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human MCM3 at the amino acid range 641-690 |
| Immunogen Region | 641-690 aa |
| Specificity | MCM3 Polyclonal Antibody detects endogenous levels of MCM3 protein. |
Additional Info
| Function | Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division (Probable). |
| Protein Name | Dna Replication Licensing Factor Mcm3Dna Polymerase Alpha Holoenzyme-Associated Protein P1P1-Mcm3Rlf Subunit BetaP102 |
| Database Links | Reactome: R-HSA-176187Reactome: R-HSA-176974Reactome: R-HSA-68867Reactome: R-HSA-68949Reactome: R-HSA-68962Reactome: R-HSA-69052 |
| Cellular Localisation | NucleusChromosomeAssociated With Chromatin Before The Formation Of Nuclei And Detaches From It As Dna Replication Progresses |
| Alternative Antibody Names | Anti-Dna Replication Licensing Factor Mcm3 antibodyAnti-Dna Polymerase Alpha Holoenzyme-Associated Protein P1 antibodyAnti-P1-Mcm3 antibodyAnti-Rlf Subunit Beta antibodyAnti-P102 antibodyAnti-MCM3 antibody |
Information sourced from Uniprot.org