| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-DNA replication licensing factor MCM3 (641-690 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC-P 1:100-300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MCM3 |
| Gene ID: | 4172 |
| Uniprot ID: | MCM3_HUMAN |
| Immunogen Region: | 641-690 aa |
| Specificity: | MCM3 Polyclonal Antibody detects endogenous levels of MCM3 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the Internal region of human MCM3 at the amino acid range 641-690 |
| Function | Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division (Probable). |
| Protein Name | Dna Replication Licensing Factor Mcm3 Dna Polymerase Alpha Holoenzyme-Associated Protein P1 P1-Mcm3 Rlf Subunit Beta P102 |
| Database Links | Reactome: R-HSA-176187 Reactome: R-HSA-176974 Reactome: R-HSA-68867 Reactome: R-HSA-68949 Reactome: R-HSA-68962 Reactome: R-HSA-69052 |
| Cellular Localisation | Nucleus Chromosome Associated With Chromatin Before The Formation Of Nuclei And Detaches From It As Dna Replication Progresses |
| Alternative Antibody Names | Anti-Dna Replication Licensing Factor Mcm3 antibody Anti-Dna Polymerase Alpha Holoenzyme-Associated Protein P1 antibody Anti-P1-Mcm3 antibody Anti-Rlf Subunit Beta antibody Anti-P102 antibody Anti-MCM3 antibody |
Information sourced from Uniprot.org