Anti-mCherry-Tag antibody (1-236 aa) [SRM5527] (STJ11107600)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal mCherry-Tag (1-236 aa) antibody for use in WB, IP and ELISA in species independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Species Independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5527 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:10000-1:40000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-600 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen Region | 1-236 aa |
| Immunogen Sequence | MVSKGEEDNMAIIKEFMRFK VHMEGSVNGHEFEIEGEGEG RPYEGTQTAKLKVTKGGPLP FAWDILSPQFMYGSKAYVKH PADIPDYLKLSFPEGFKWER VMNFEDGGVVTVTQDSSLQD GEFIYKVKLRGTNFPSDGPV MQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDA EVKTTYKAKKPVQLPGAYNV NIKLDITSHNEDYTIVEQYE RAEGRHSTGGMDELYK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of Discosoma MCherry fluorescent proteinm. |
Additional Info
| Background | Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification. |
Information sourced from Uniprot.org