Anti-mCherry antibody (STJ140213)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant peptide produced in E. coli.
General Information
| Short Description | Goat polyclonal mCherry antibody for use in WB, IF, IHC-P, IHC-F and IEM in rfp, dsred, tdtomato and mcherry samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/IHC-P/IHC-F/IEM |
| Host | Goat |
| Reactivity | RFP/DsRed/tdTomato/mCherry |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 2 mg/mL |
| Purification | Epitope affinity purified |
| Dilution Range | WB 1:500-1:5000IHC-F 1:50-1:500IHC-P 1:50-1:500IF 1:50-1:500IEM 1:50-1:500 |
| Formulation | NaHCO3 100 mM pH 9. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Immunogen | Purified recombinant peptide produced in E. coli. |
| Immunogen Sequence | MVSKGEEDNMAIIKEFMRFK VSKGEEDNMAIIKEFMRFKV SKGEEDNMAIIKEFMRFKVH KGEEDNMAIIKEFMRFKVHM GEEDNMAIIKEFMRFKVHME EEDNMAIIKEFMRFKVHMEG EDNMAIIKEFMRFKVHMEGS DNMAIIKEFMRFKVHMEGSV NMAIIKEFMRFKVHMEGSVN MAIIKEFMRFKVHMEGSVNG AIIKEFMRFKVHMEGSVNGH IIKEFMRFKVHMEGSVNGH |
| Specificity | In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein). |