Anti-MBNL1 antibody (280-380 aa) (STJ11107046)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MBNL1 (280-380 aa) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MBNL1 |
| Gene ID | 4154 |
| Uniprot ID | MBNL1_HUMAN |
| Immunogen Region | 280-380 aa |
| Immunogen Sequence | PLEATFDLGIPQAVLPPLPK RPALEKTNGATAVFNTGIFQ YQQALANMQLQQHTAFLPPV PMVHGATPATVSAATTSATS VPFAATATANQIPIISAEHL T |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 280-380 of human MBNL1 (NP_997176.1). |
Additional Info
| Tissue Specificity | Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. Weakly expressed in other tissues (at protein level). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Function | Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. Together with RNA binding proteins RBPMS and RBFOX2, activates vascular smooth muscle cells alternative splicing events. Regulates NCOR2 alternative splicing. |
| Protein Name | Muscleblind-Like Protein 1Triplet-Expansion Rna-Binding Protein |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCytoplasmic GranuleLocalized With Ddx1Tial1 And Ybx1 In Stress Granules Upon StressLocalized In The Cytoplasm Of Multinucleated MyotubesColocalizes With Nuclear Foci Of Retained Expanded-Repeat Transcripts In Myotubes From Patients Affected By Myotonic Dystrophy |
| Alternative Antibody Names | Anti-Muscleblind-Like Protein 1 antibodyAnti-Triplet-Expansion Rna-Binding Protein antibodyAnti-MBNL1 antibodyAnti-EXP antibodyAnti-KIAA0428 antibodyAnti-MBNL antibody |
Information sourced from Uniprot.org