• Western blot analysis of extracts of various cell lines, using MARS antibody (STJ111979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using MARS antibody (STJ111979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MARS antibody (1-270) (STJ111979)

SKU:
STJ111979

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MARS (1-270) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MARS1
Gene ID: 4141
Uniprot ID: SYMC_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MARS (NP_004981.2).
Immunogen Sequence: MRLFVSDGVPGCLPVLAAAG RARGRAEVLISTVGPEDCVV PFLTRPKVPVLQLDSGNYLF STSAICRYFFLLSGWEQDDL TNQWLEWEATELQPALSAAL YYLVVQGKKGEDVLGSVRRA LTHIDHSLSRQNCPFLAGET ESLADIVLWGALYPLLQDPA YLPEELSALHSWFQTLSTQE PCQRAAETVLKQQGVLALRP YLQKQPQPSPAEGRAVTNEP EEEELATLSEEEIAMAVTA
Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Plays a role in the synthesis of ribosomal RNA in the nucleolus.
Protein Name Methionine--Trna Ligase - Cytoplasmic
Methionyl-Trna Synthetase
Metrs
Database Links Reactome: R-HSA-2408522
Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Nucleolus
Localizes To The Nucleolus In Proliferative Cells But Disappears In Quiescent Cells
Alternative Antibody Names Anti-Methionine--Trna Ligase - Cytoplasmic antibody
Anti-Methionyl-Trna Synthetase antibody
Anti-Metrs antibody
Anti-MARS1 antibody
Anti-MARS antibody

Information sourced from Uniprot.org

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