• Immunohistochemistry analysis of MAPK1/MAPK3 in paraffin-embedded rat lung using MAPK1/MAPK3 Rabbit polyclonal antibody (STJ119426) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MAPK1/MAPK3 in paraffin-embedded mouse heart using MAPK1/MAPK3 Rabbit polyclonal antibody (STJ119426) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MAPK1/MAPK3 in paraffin-embedded human urothelial carcinoma using MAPK1/MAPK3 Rabbit polyclonal antibody (STJ119426) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MAPK1/MAPK3 Rabbit polyclonal antibody (STJ119426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAPK1/MAPK3 antibody (200-300) (STJ119426)

SKU:
STJ119426

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAPK1/MAPK3 (200-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MAPK1/MAPK3ERK1/2 (P27361/P28482).
Immunogen Sequence: FLTEYVATRWYRAPEIMLNS KGYTKSIDIWSVGCILAEML SNRPIFPGKHYLDQLNHILG ILGSPSQEDLNCIINMKARN YLQSLPSKTKVAWAKLFPKS D/LNSKGYTKSIDIWSVGCI LAEMLSNRPIFPGKHYLDQL NHILGILGSPSQEDLNCIIN LKARNYLLSLPHKNKVPWNR LFPNADSKALDLLDKMLTFN PHK
Background Predicted to enable endoribonuclease activity and mRNA binding activity. Predicted to be involved in RNA phosphodiester bond hydrolysis, endonucleolytic. Predicted to be active in cytoplasmic ribonucleoprotein granule and nucleus.

Information sourced from Uniprot.org

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