| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MAP4K2 (551-820) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAP4K2 |
| Gene ID: |
5871 |
| Uniprot ID: |
M4K2_HUMAN |
| Immunogen Region: |
551-820 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 551-820 of human MAP4K2 (NP_004570.2). |
| Immunogen Sequence: |
WAHDLPGLFEQRRLQQQVPL SIPTNRLTQRIIPRRFALST KIPDTKGCLQCRVVRNPYTG ATFLLAALPTSLLLLQWYEP LQKFLLLKNFSSPLPSPAGM LEPLVLDGKELPQVCVGAEG PEGPGCRVLFHVLPLEAGLT PDILIPPEGIPGSAQQVIQV DRDTILVSFERCVRIVNMQG EPTATLAPELTFDFPIETVV CLQDSVLAFWSHGMQGRSLD TNEVTQEITDETRIFRVLG |
| Tissue Specificity | Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined. |
| Post Translational Modifications | Polyubiquitinated through 'Lys-48'-polyubiquitin chains, allowing proteasomal turnover. Ubiquitination requires the kinase activity of MAP4K2/GCK. Autophosphorylated in response to tumor necrosis factor (TNF), endotoxins or pro-inflammatory stimuli. Autophosphorylation leads to activation. |
| Function | Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2B Lymphocyte Serine/Threonine-Protein KinaseGerminal Center KinaseGc KinaseMapk/Erk Kinase Kinase Kinase 2Mek Kinase Kinase 2Mekkk 2Rab8-Interacting Protein |
| Cellular Localisation | CytoplasmBasolateral Cell MembranePeripheral Membrane ProteinGolgi Apparatus Membrane |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 antibodyAnti-B Lymphocyte Serine/Threonine-Protein Kinase antibodyAnti-Germinal Center Kinase antibodyAnti-Gc Kinase antibodyAnti-Mapk/Erk Kinase Kinase Kinase 2 antibodyAnti-Mek Kinase Kinase 2 antibodyAnti-Mekkk 2 antibodyAnti-Rab8-Interacting Protein antibodyAnti-MAP4K2 antibodyAnti-GCK antibodyAnti-RAB8IP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
