• Western blot analysis of various lysates using MAP4K2 Rabbit polyclonal antibody (STJ119617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAP4K2 antibody (551-820) (STJ119617)

SKU:
STJ119617

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAP4K2 (551-820) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP4K2
Gene ID: 5871
Uniprot ID: M4K2_HUMAN
Immunogen Region: 551-820
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 551-820 of human MAP4K2 (NP_004570.2).
Immunogen Sequence: WAHDLPGLFEQRRLQQQVPL SIPTNRLTQRIIPRRFALST KIPDTKGCLQCRVVRNPYTG ATFLLAALPTSLLLLQWYEP LQKFLLLKNFSSPLPSPAGM LEPLVLDGKELPQVCVGAEG PEGPGCRVLFHVLPLEAGLT PDILIPPEGIPGSAQQVIQV DRDTILVSFERCVRIVNMQG EPTATLAPELTFDFPIETVV CLQDSVLAFWSHGMQGRSLD TNEVTQEITDETRIFRVLG
Tissue Specificity Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.
Post Translational Modifications Polyubiquitinated through 'Lys-48'-polyubiquitin chains, allowing proteasomal turnover. Ubiquitination requires the kinase activity of MAP4K2/GCK. Autophosphorylated in response to tumor necrosis factor (TNF), endotoxins or pro-inflammatory stimuli. Autophosphorylation leads to activation.
Function Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2
B Lymphocyte Serine/Threonine-Protein Kinase
Germinal Center Kinase
Gc Kinase
Mapk/Erk Kinase Kinase Kinase 2
Mek Kinase Kinase 2
Mekkk 2
Rab8-Interacting Protein
Cellular Localisation Cytoplasm
Basolateral Cell Membrane
Peripheral Membrane Protein
Golgi Apparatus Membrane
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 antibody
Anti-B Lymphocyte Serine/Threonine-Protein Kinase antibody
Anti-Germinal Center Kinase antibody
Anti-Gc Kinase antibody
Anti-Mapk/Erk Kinase Kinase Kinase 2 antibody
Anti-Mek Kinase Kinase 2 antibody
Anti-Mekkk 2 antibody
Anti-Rab8-Interacting Protein antibody
Anti-MAP4K2 antibody
Anti-GCK antibody
Anti-RAB8IP antibody

Information sourced from Uniprot.org

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