• Immunoprecipitation analysis of Hela cell lysates using MKK3/6 mouse mAb.
  • Western blot analysis of extracts from PC12, C6, Ramos, Jurkat and Hela cell lysates using MKK3/6 mouse mAb (1:1000 diluted).Predicted band size:40KDa.Observed band size:40KDa.

Anti-MAP2K6 antibody [8A11-G11-G11] (STJ99282)

SKU:
STJ99282

Current Stock:
Host: Mouse
Applications: WB/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Dual specificity mitogen-activated protein kinase kinase 6 is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: 8A11-G11-G11
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2K6
Gene ID: 5608
Uniprot ID: MP2K6_HUMAN
Specificity: This antibody detects endogenous levels of MKK3 and MKK6 and does not cross-react with related proteins.
Immunogen: Purified recombinant human MKK6 protein fragments expressed in E.coli.
Post Translational Modifications Weakly autophosphorylated. Phosphorylated at Ser-207 and Thr-211 by the majority of M3Ks, such as MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2. (Microbial infection) Acetylation of Ser-207 and Thr-211 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction.and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 6
Map Kinase Kinase 6
Mapkk 6
Mapk/Erk Kinase 6
Mek 6
Stress-Activated Protein Kinase Kinase 3
Sapk Kinase 3
Sapkk-3
Sapkk3
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-2559580
Reactome: R-HSA-450302
Reactome: R-HSA-5210891 P52564-1
Reactome: R-HSA-525793
Reactome: R-HSA-6811555
Reactome: R-HSA-9020702
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Binds To Microtubules
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 6 antibody
Anti-Map Kinase Kinase 6 antibody
Anti-Mapkk 6 antibody
Anti-Mapk/Erk Kinase 6 antibody
Anti-Mek 6 antibody
Anti-Stress-Activated Protein Kinase Kinase 3 antibody
Anti-Sapk Kinase 3 antibody
Anti-Sapkk-3 antibody
Anti-Sapkk3 antibody
Anti-MAP2K6 antibody
Anti-MEK6 antibody
Anti-MKK6 antibody
Anti-PRKMK6 antibody
Anti-SKK3 antibody

Information sourced from Uniprot.org

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