• Western blot analysis of various lysates using MAML1 Rabbit polyclonal antibody (STJ119287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-MAML1 antibody (1-300) (STJ119287)

SKU:
STJ119287

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAML1 (1-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAML1
Gene ID: 9794
Uniprot ID: MAML1_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MAML1 (NP_055572.1).
Immunogen Sequence: MVLPTCPMAEFALPRHSAVM ERLRRRIELCRRHHSTCEAR YEAVSPERLELERQHTFALH QRCIQAKAKRAGKHRQPPAA TAPAPAAPAPRLDAADGPEH GRPATHLHDTVKRNLDSATS PQNGDQQNGYGDLFPGHKKT RREAPLGVAISSNGLPPASP LGQSDKPSGADALQSSGKHS LGLDSLNKKRLADSSLHLNG GSNPSESFPLSLNKELKQEP VEDLPCMITGTVGSISQSN
Tissue Specificity Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
Function Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
Protein Name Mastermind-Like Protein 1
Mam-1
Database Links Reactome: R-HSA-1912408
Reactome: R-HSA-210744
Reactome: R-HSA-2122947
Reactome: R-HSA-2197563
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-350054
Reactome: R-HSA-8941856
Reactome: R-HSA-9013508
Reactome: R-HSA-9013695
Reactome: R-HSA-9793380
Cellular Localisation Nucleus Speckle
Nuclear
In A Punctate Manner
Alternative Antibody Names Anti-Mastermind-Like Protein 1 antibody
Anti-Mam-1 antibody
Anti-MAML1 antibody
Anti-KIAA0200 antibody

Information sourced from Uniprot.org

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