• HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with STJ70705 (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with STJ70705 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Anti-Makorin antibody (Internal) (STJ70705)

SKU:
STJ70705-100

€379.38
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Makorin (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MKRN1
Gene ID: 23608
Uniprot ID: MKRN1_HUMAN
Immunogen Region: Internal
Accession Number: NP_038474.1; NP_001138597.1
Specificity: This antibody may cross react with MKRN4 (52.9kDa).
Immunogen Sequence: VTKEESEKEQTAVET
Post Translational Modifications Auto-ubiquitinated.which leads to proteasomal degradation.
Function E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
Protein Name E3 Ubiquitin-Protein Ligase Makorin-1
Ring Finger Protein 61
Ring-Type E3 Ubiquitin Transferase Makorin-1
Database Links Reactome: R-HSA-198323
Reactome: R-HSA-8948751
Reactome: R-HSA-983168
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Makorin-1 antibody
Anti-Ring Finger Protein 61 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Makorin-1 antibody
Anti-MKRN1 antibody
Anti-RNF61 antibody

Information sourced from Uniprot.org

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