Anti-MAGEL2 antibody (STJA0004863)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide of human MAGEL2
General Information
| Short Description | Rabbit polyclonal anti-Mage-Like Protein 2 for use in IHC and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.8 mg/mL |
| Purification | Affinity purification |
| Dilution Range | IHC 1:50-1:200 |
| Formulation | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | MAGEL2 |
| Gene ID | 54551 |
| Uniprot ID | MAGL2_HUMAN |
| Immunogen | Synthetic peptide of human MAGEL2 |
Additional Info
| Tissue Specificity | Expressed in placenta, fetal and adult brain. Not detected in heart and small intestine, very low levels in fibroblasts. Not expressed in brain of a Prader-Willi patient. |
| Function | Probably enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, possibly through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Acts as a regulator of retrograde transport via its interaction with VPS35. Recruited to retromer-containing endosomes and promotes the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1 together with TRIM27, leading to promote endosomal F-actin assembly. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Significantly promotes the cytoplasmic accumulation of CLOCK. |
| Protein Name | Mage-Like Protein 2Necdin-Like Protein 1Protein Nm15 |
| Database Links | |
| Cellular Localisation | Early EndosomeCytoplasmNucleusRecruited To Retromer-Containing Endosomes Via Interaction With Vps35Colocalizes With Clock And Bmal1 In The CytoplasmAnd With Per2 In The Cytoplasm And Nucleus |
| Alternative Antibody Names | Anti-Mage-Like Protein 2 antibodyAnti-Necdin-Like Protein 1 antibodyAnti-Protein Nm15 antibodyAnti-MAGEL2 antibodyAnti-NDNL1 antibody |
Information sourced from Uniprot.org