Host: |
Mouse |
Applications: |
FC/WB/IHC/IP |
Reactivity: |
Human |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Mouse monoclonal antibody anti-Lymphocytic Marker is suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunoprecipitation research applications. |
Clonality: |
Monoclonal |
Clone ID: |
IPO-74 |
Conjugation: |
Unconjugated |
Isotype: |
IgG1k |
Formulation: |
PBS with 0.02% Sodium Azide. |
Purification: |
Affinity purified from tissue culture. |
Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
Dilution Range: |
Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunoblotting (1, 0-2, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH6, 0, for 10-20 min f |
Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
Immunogen: |
A BALB/c mouse was mouse with human cell line HL-60. Fusion partner: P3-X63-Ag8.653. |
Background | IPO-74 reacts with human leukemia cell line HL-60 and shows a band at MW 127 kDa (reduced) both in immunoprecipitation and Western blotting. In peripheral blood only subsets of B cells, T cells, neutrophilic and eosinophilic granulocytes are positive. All monocytes are positive. Cell lines U937 and K562 are positive, while Daudy, Raji, Jurkat and Molt-4 are negative, as are IL2-dependent T cell lines/clones. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance