Anti-Lymphocytic Marker antibody [IPO-74] (STJ16101001)

SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenA BALB/c mouse was mouse with human cell line HL-60. Fusion partner: P3-X63-Ag8.653.
STJ16101001
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General Information

Short DescriptionMouse monoclonal anti-Lymphocytic Marker for use in FC, WB, IHC and IP in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsFC/WB/IHC/IP
HostMouse
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDIPO-74
IsotypeIgG1k
ConjugationUnconjugated
ConcentrationCan be provided as 100 ยตg/mL, 500 ยตg/mL or 1mg/mL.
PurificationAffinity purified from tissue culture.
Dilution RangeFlow cytometry (0, 5-1, 0 ยตg/million cells in 0, 1 ml). Immunoblotting (1, 0-2, 0 ยตg/ml). Immunohistology (1-2 ยตg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH6, 0, for 10-20 min f
FormulationPBS with 0.02% Sodium Azide.
Storage InstructionStore for up to 1 year at 2-8ยฐC upon receipt.

Target Information

ImmunogenA BALB/c mouse was mouse with human cell line HL-60. Fusion partner: P3-X63-Ag8.653.

Additional Info

Background IPO-74 reacts with human leukemia cell line HL-60 and shows a band at MW 127 kDa (reduced) both in immunoprecipitation and Western blotting. In peripheral blood only subsets of B cells, T cells, neutrophilic and eosinophilic granulocytes are positive. All monocytes are positive. Cell lines U937 and K562 are positive, while Daudy, Raji, Jurkat and Molt-4 are negative, as are IL2-dependent T cell lines/clones.

Information sourced from Uniprot.org

Citations

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