Anti-Lymphocytic Marker antibody [IPO-74] (STJ16101001)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenA BALB/c mouse was mouse with human cell line HL-60. Fusion partner: P3-X63-Ag8.653.
General Information
| Short Description | Mouse monoclonal anti-Lymphocytic Marker for use in FC, WB, IHC and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/WB/IHC/IP |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | IPO-74 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 ยตg/mL, 500 ยตg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow cytometry (0, 5-1, 0 ยตg/million cells in 0, 1 ml). Immunoblotting (1, 0-2, 0 ยตg/ml). Immunohistology (1-2 ยตg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH6, 0, for 10-20 min f |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8ยฐC upon receipt. |
Target Information
| Immunogen | A BALB/c mouse was mouse with human cell line HL-60. Fusion partner: P3-X63-Ag8.653. |
Additional Info
| Background | IPO-74 reacts with human leukemia cell line HL-60 and shows a band at MW 127 kDa (reduced) both in immunoprecipitation and Western blotting. In peripheral blood only subsets of B cells, T cells, neutrophilic and eosinophilic granulocytes are positive. All monocytes are positive. Cell lines U937 and K562 are positive, while Daudy, Raji, Jurkat and Molt-4 are negative, as are IL2-dependent T cell lines/clones. |
Information sourced from Uniprot.org