| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | LYAR |
| Gene ID: | 55646 |
| Uniprot ID: | LYAR_HUMAN |
| Immunogen Region: | 10-160 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 10-160 of human LYAR (NP_060286.1). |
| Immunogen Sequence: | GESVKKIQVEKHVSVCRNCE CLSCIDCGKDFWGDDYKNHV KCISEDQKYGGKGYEGKTHK GDIKQQAWIQKISELIKRPN VSPKVRELLEQISAFDNVPR KKAKFQNWMKNSLKVHNESI LDQVWNIFSEASNSEPVNKE QDQRPLHPVAN |
| Tissue Specificity | Predominantly expressed in testis. |
| Function | Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs. Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression. In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA. Preferentially binds to a DNA motif containing 5'-GGTTAT-3'. Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity. In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines. Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells. Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal. |
| Protein Name | Cell Growth-Regulating Nucleolar Protein |
| Cellular Localisation | Nucleus Nucleolus Cytoplasm Cell Projection Cilium Photoreceptor Outer Segment Component Of Pre-Ribosomal Particles Including Pre-40s Pre-60s And Pre-90s Associated With Cytoplasmic Ribosomes But Not Polysomes As A Component Of The 60s Subunit In The Retina Predominantly Expressed In Photoreceptor Outer Segments In The Nucleolus Colocalizes With Nucleolin/Ncl Therefore May Reside In The Dense Fibrillar Component (Dfc) |
| Alternative Antibody Names | Anti-Cell Growth-Regulating Nucleolar Protein antibody Anti-LYAR antibody Anti-PNAS-5 antibody |
Information sourced from Uniprot.org