| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LYAR (10-160) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LYAR |
| Gene ID: |
55646 |
| Uniprot ID: |
LYAR_HUMAN |
| Immunogen Region: |
10-160 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 10-160 of human LYAR (NP_060286.1). |
| Immunogen Sequence: |
GESVKKIQVEKHVSVCRNCE CLSCIDCGKDFWGDDYKNHV KCISEDQKYGGKGYEGKTHK GDIKQQAWIQKISELIKRPN VSPKVRELLEQISAFDNVPR KKAKFQNWMKNSLKVHNESI LDQVWNIFSEASNSEPVNKE QDQRPLHPVAN |
| Tissue Specificity | Predominantly expressed in testis. |
| Function | Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs. Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression. In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA. Preferentially binds to a DNA motif containing 5'-GGTTAT-3'. Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity. In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines. Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells. Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal. |
| Protein Name | Cell Growth-Regulating Nucleolar Protein |
| Cellular Localisation | NucleusNucleolusCytoplasmCell ProjectionCiliumPhotoreceptor Outer SegmentComponent Of Pre-Ribosomal ParticlesIncluding Pre-40sPre-60s And Pre-90sAssociated With Cytoplasmic RibosomesBut Not PolysomesAs A Component Of The 60s SubunitIn The RetinaPredominantly Expressed In Photoreceptor Outer SegmentsIn The NucleolusColocalizes With Nucleolin/NclTherefore May Reside In The Dense Fibrillar Component (Dfc) |
| Alternative Antibody Names | Anti-Cell Growth-Regulating Nucleolar Protein antibodyAnti-LYAR antibodyAnti-PNAS-5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and LYAR knockout (KO) 293T cells, using [KO Validated] LYAR Rabbit polyclonal antibody (STJ11100776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89712/358194/STJ11100776_1__10553.1713122510.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LYAR knockout (KO) 293T cells, using [KO Validated] LYAR Rabbit polyclonal antibody (STJ11100776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
